Homology threading to generate RNA polymerase structures

Protein Expr Purif. 2018 Jul:147:13-16. doi: 10.1016/j.pep.2018.02.002. Epub 2018 Feb 11.

Abstract

Homology threading is a powerful technology for generating structural models based on homologous structures. Here we use threading to generate four complex RNA polymerase models. The models appear to be as useful as x-ray crystal structures or cryo-electron microscopy structures to support research projects.

Keywords: Drosophila melanogaster RNA polymerase II preinitiation complex; Homo sapiens RNA polymerase I and III; Homology modeling.

Publication types

  • Review

MeSH terms

  • Animals
  • Computational Biology / methods*
  • Cryoelectron Microscopy / methods
  • Crystallography, X-Ray
  • DNA-Directed RNA Polymerases / chemistry*
  • DNA-Directed RNA Polymerases / metabolism
  • DNA-Directed RNA Polymerases / ultrastructure
  • Humans
  • Models, Molecular*
  • Nucleic Acid Conformation
  • Nucleic Acids / chemistry
  • Nucleic Acids / metabolism
  • Nucleic Acids / ultrastructure
  • Protein Conformation*

Substances

  • Nucleic Acids
  • DNA-Directed RNA Polymerases