Abstract
Sesame is an important oilseed crop, which has been used as a traditional health food to ameliorate the prevention of various diseases. We evaluated the changes in the anti-allergic activities of sesame by bioconversion. SDS-PAGE of non-fermented sesame proteins showed major allergen bands, while that of fermented sesame showed only a few protein bands. Additionally, we investigated the effectiveness of fermented sesame by bioconversion in tumor necrosis factor-α (TNF-α)- and interferon-γ (IFN-γ)-induced HaCaT cells. In HaCaT cells, fermented sesame inhibited the mRNA expression of interleukin-6 (IL-6) and interleukin-1β (IL-1β), thymus and macrophage-derived chemokine (MDC/CCL22), activation-regulated chemokine (TARC/CCL17), and intercellular adhesion molecule-1 (ICAM-1). Moreover, fermented sesame inhibited the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 1 (STAT1). Fermented sesame exerts anti-allergic effects by suppressing the expression of chemokines and cytokines via blockade of NF-κB and STAT1 activation.
Keywords:
Sesamum indicum L.; anti-allergic; atopic dermatitis; bioconversion; skin inflammation.
MeSH terms
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Agaricales
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Allergens / adverse effects*
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Allergens / analysis
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Allergens / metabolism
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Cell Line
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Chemokines / antagonists & inhibitors
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Chemokines / genetics
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Chemokines / metabolism
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Crops, Agricultural / adverse effects
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Crops, Agricultural / chemistry
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Crops, Agricultural / growth & development
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Crops, Agricultural / microbiology
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Cytokines / antagonists & inhibitors*
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Cytokines / genetics
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Cytokines / metabolism
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Dermatitis, Atopic / etiology
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Dermatitis, Atopic / immunology
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Dermatitis, Atopic / prevention & control
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Fermentation
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Fermented Foods / adverse effects
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Fermented Foods / analysis*
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Fermented Foods / microbiology
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Food Handling
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Food Hypersensitivity / etiology
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Food Hypersensitivity / immunology
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Food Hypersensitivity / prevention & control
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Fruiting Bodies, Fungal
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Gene Expression Regulation, Neoplastic
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Humans
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Keratinocytes / immunology
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Keratinocytes / metabolism*
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Plant Proteins, Dietary / adverse effects*
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Plant Proteins, Dietary / analysis
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Plant Proteins, Dietary / metabolism
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Republic of Korea
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Seeds / adverse effects
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Seeds / chemistry*
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Seeds / growth & development
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Seeds / microbiology
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Sesamum / adverse effects
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Sesamum / chemistry*
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Sesamum / growth & development
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Sesamum / microbiology
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Shiitake Mushrooms / isolation & purification
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Shiitake Mushrooms / metabolism
Substances
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Allergens
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Chemokines
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Cytokines
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Plant Proteins, Dietary