One dimensional (1D) NiFe2O4 nanofibers have been successfully fabricated using single-nozzle electrospinning technique and subsequent calcination process through adjusting heat-treatment parameters. The calcination procedure plays a key role for the formation of nanofibers with different morphologies. The lithium storage properties of NiFe2O4 nanofibers were evaluated by galvanostatic cycling studies in half-cell configuration. Furthermore, their photocatalytic property under vis-light irradiation with H2O2 addition is investigated at room temperature. As-synthesized nanofibers with regular 1D nanostructure with large surface area and high crystallinity calcined at 600 °C exhibited fine lithium storage with excellent cycling and rate performance, as well as excellent photocatalytic performance with 97% degradation proportion of methylene blue after 30 min. This is ascribed to NiFe2O4 absorbed the photon energy and generated electron-hole pairs under vis-light irradiation.