Sequence-specific DNA binding Pyrrole-imidazole polyamides and their applications

Yusuke Kawamoto; Toshikazu Bando; Hiroshi Sugiyama

doi:10.1016/j.bmc.2018.01.026

Sequence-specific DNA binding Pyrrole-imidazole polyamides and their applications

Bioorg Med Chem. 2018 May 1;26(8):1393-1411. doi: 10.1016/j.bmc.2018.01.026. Epub 2018 Feb 1.

Authors

Yusuke Kawamoto¹, Toshikazu Bando², Hiroshi Sugiyama³

Affiliations

¹ Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
² Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan. Electronic address: bando@kuchem.kyoto-u.ac.jp.
³ Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan; Institute for Integrated Cell-Material Science (iCeMS), Kyoto University, Sakyo, Kyoto 606-8501, Japan. Electronic address: hs@kuchem.kyoto-u.ac.jp.

PMID: 29439914
DOI: 10.1016/j.bmc.2018.01.026

Abstract

Pyrrole-imidazole polyamides (Py-Im polyamides) are cell-permeable compounds that bind to the minor groove of double-stranded DNA in a sequence-specific manner without causing denaturation of the DNA. These compounds can be used to control gene expression and to stain specific sequences in cells. Here, we review the history, structural variations, and functional investigations of Py-Im polyamides.

Keywords: Cellular imaging; Gene regulation; Minor groove binder; Pyrrole−imidazole polyamides.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
DNA / chemistry
DNA / drug effects*
DNA / genetics
Gene Expression Regulation / drug effects
Humans
Imidazoles / chemistry
Imidazoles / pharmacology*
Molecular Structure
Nylons / chemistry
Nylons / pharmacology*
Pyrroles / chemistry
Pyrroles / pharmacology*
Transcriptional Activation / drug effects

Substances

Imidazoles
Nylons
Pyrroles
DNA