Potential roles for glucagon-like peptide-17-36 amide and cholecystokinin in anorectic response to the trichothecene mycotoxin T-2 toxin

Wenda Wu; Kun Sheng; Xinglian Xu; Haibin Zhang; Guanghong Zhou

doi:10.1016/j.ecoenv.2018.02.003

Potential roles for glucagon-like peptide-1_7-36 amide and cholecystokinin in anorectic response to the trichothecene mycotoxin T-2 toxin

Ecotoxicol Environ Saf. 2018 May 30:153:181-187. doi: 10.1016/j.ecoenv.2018.02.003. Epub 2018 Feb 10.

Authors

Wenda Wu¹, Kun Sheng², Xinglian Xu³, Haibin Zhang⁴, Guanghong Zhou⁵

Affiliations

¹ College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; Key Laboratory of Meat Processing, Key Lab of Meat Processing and Quality Control, Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
² College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
³ Key Laboratory of Meat Processing, Key Lab of Meat Processing and Quality Control, Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
⁴ College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; Key Laboratory of Meat Processing, Key Lab of Meat Processing and Quality Control, Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: zhhbnau@163.com.
⁵ Key Laboratory of Meat Processing, Key Lab of Meat Processing and Quality Control, Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: guanghong.zhou@hotmail.com.

PMID: 29433086
DOI: 10.1016/j.ecoenv.2018.02.003

Abstract

Anorexia is a hallmark of animal and human exposed to T-2 toxin, a most poisonous trichothecene mycotoxins contaminating various cereal grains including wheat, corn and barley. Although this adverse effect has been well characterized in several animal species, the underlying mechanisms are unclear. The goal for this study was to elucidate the roles of two gut satiety hormones, glucagon-like peptide-1_7-36 amide (GLP-1) and cholecystokinin (CCK) in T-2 toxin-evoked anorectic response using a mouse anorexia bioassay. Elevations of plasma GLP-1 and CCK significantly corresponded to anorexia induction by T-2 toxin. Direct administration of exogenous GLP-1 and CCK markedly evoked anorectic responses similar to T-2 toxin. The GLP-1 receptor (GLP-1R) antagonist Exendin9-39 dose-dependently cause attenuation of both GLP-1- and T-2 toxin-induced anorectic responses. Pretreatment with the CCK1 receptor (CCK1R) antagonist SR 27897 and CCK2 receptor (CCK2R) antagonist L-365,260 attenuated anorexia induction by both CCK- and T-2 toxin in a dose dependent manner. Taken together, our findings suggest that both GLP-1 and CCK play contributory roles in T-2 toxin-induced anorexia.

Keywords: Anorexia; Cholecystokinin; Glucagon-like peptide-1(7–36) amide; Gut satiety hormone; Mycotoxin; T-2 toxin.

MeSH terms

Animals
Anorexia / blood
Anorexia / chemically induced*
Cholecystokinin / administration & dosage
Cholecystokinin / blood*
Dose-Response Relationship, Drug
Eating / drug effects
Female
Glucagon-Like Peptide 1 / administration & dosage
Glucagon-Like Peptide 1 / blood*
Mice
Mice, Inbred Strains
Peptide Fragments / administration & dosage
Peptide Fragments / blood*
T-2 Toxin / toxicity*

Substances

Peptide Fragments
glucagon-like peptide 1 (7-36)
Glucagon-Like Peptide 1
Cholecystokinin
T-2 Toxin