Knockdown of ARL4C inhibits osteogenic differentiation of human adipose-derived stem cells through disruption of the Wnt signaling pathway

Biochem Biophys Res Commun. 2018 Feb 26;497(1):256-263. doi: 10.1016/j.bbrc.2018.02.066. Epub 2018 Feb 9.

Abstract

ADP-ribosylation factor-like 4C (ARL4C) has been shown to play an important role in cholesterol secretion, microtubule dynamics, and cell morphological changes. However, its role in osteogenesis has not been explored. In this study, we found that ARL4C is downregulated during the osteogenic differentiation of human adipose derived stem cells (hASCs). Knockdown of ARL4C suppresses osteogenesis of hASCs in vitro and in vivo. We demonstrate that ARL4C knockdown likely attenuates osteogenesis of hASCs through inhibition of the Wnt signaling pathway. These results provide new insights into the mechanisms of osteogenic differentiation and provide a potential molecular target for bone tissue engineering.

Keywords: ARL4C; Human adipose-derived stem cells; Osteogenic differentiation; Wnt signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factors / genetics
  • ADP-Ribosylation Factors / metabolism*
  • Adipocytes / cytology*
  • Adipocytes / physiology
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Gene Expression Regulation, Developmental / physiology
  • Gene Knockdown Techniques
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / physiology*
  • Osteogenesis / physiology*
  • Wnt Signaling Pathway / physiology*

Substances

  • ADP-Ribosylation Factors
  • ARL4C protein, human