A New Ratiometric Lysosomal Copper(II) Fluorescent Probe To Map a Dynamic Metallome in Live Cells

Maria Laura Giuffrida; Giuseppe Trusso Sfrazzetto; Cristina Satriano; Stefania Zimbone; Gaetano A Tomaselli; Agata Copani; Enrico Rizzarelli

doi:10.1021/acs.inorgchem.7b02720

A New Ratiometric Lysosomal Copper(II) Fluorescent Probe To Map a Dynamic Metallome in Live Cells

Inorg Chem. 2018 Mar 5;57(5):2365-2368. doi: 10.1021/acs.inorgchem.7b02720. Epub 2018 Feb 12.

Authors

Maria Laura Giuffrida¹, Giuseppe Trusso Sfrazzetto, Cristina Satriano, Stefania Zimbone¹, Gaetano A Tomaselli, Agata Copani¹, Enrico Rizzarelli¹

Affiliation

¹ Institute of Biostructures and Bioimages, National Council of Research , Via P. Gaifami 18 , Catania 95126 , Italy.

PMID: 29431435
DOI: 10.1021/acs.inorgchem.7b02720

Abstract

We synthesized a new ratiometric fluorescent Cu²⁺ probe, bearing a morpholine moiety for selective binding to lysosomes and two picolylamine arms for the specific chelation of divalent copper ions. The probe capability to detect lysosomal Cu²⁺ was demonstrated in human differentiated neuroblastoma cells by confocal microscopy.

MeSH terms

Cell Differentiation
Chelating Agents / chemical synthesis
Chelating Agents / chemistry*
Copper / chemistry*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Humans
Lysosomes / chemistry*
Microscopy, Confocal
Morpholines / chemistry
Neuroblastoma / pathology*
Optical Imaging*
Picolines / chemistry

Substances

Chelating Agents
Fluorescent Dyes
Morpholines
Picolines
Copper
morpholine