The main sources of soil contamination by oil products in the Dnepropetrovsk region are oil depots, gas stations and all types of transport that use and transport oil. The diesel fuel was found to be the one of the priority multicomponent components of the petroleum products, so it there was occurred the necessity for the hygienic regulation of the oil component in order to monitor a multicomponent composition ofpetroleum products in the study of complex influence of environmental factors on health population. In the study of the effect of various concentrations of diesel fuel on the number of total bacterial count (TBC) as the threshold for general sanitary indicator of hazard, there was recommended the concentration of4000 mg/kg, which oppressed the vital activity of soil microorganisms by 50-27.3% for the first 2 weeks of the laboratory experiment.