Core-shell structured magnetic metal-organic framework composites for highly selective detection of N-glycopeptides based on boronic acid affinity chromatography

Yiqin Xie; Qianjing Liu; Yan Li; Chunhui Deng

doi:10.1016/j.chroma.2018.02.013

Core-shell structured magnetic metal-organic framework composites for highly selective detection of N-glycopeptides based on boronic acid affinity chromatography

J Chromatogr A. 2018 Mar 9:1540:87-93. doi: 10.1016/j.chroma.2018.02.013. Epub 2018 Feb 6.

Authors

Yiqin Xie¹, Qianjing Liu¹, Yan Li², Chunhui Deng³

Affiliations

¹ The Fifth People's Hospital of Shanghai, Department of Chemistry, Institutes of Biomedical Sciences, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China.
² Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai, 201206, China. Electronic address: yanli@fudan.edu.cn.
³ The Fifth People's Hospital of Shanghai, Department of Chemistry, Institutes of Biomedical Sciences, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China. Electronic address: chdeng@fudan.edu.cn.

PMID: 29429745
DOI: 10.1016/j.chroma.2018.02.013

Abstract

Boronic acid affinity chromatography (BAAC) is one of the most significant methods in glycoproteomics research due to its low bias towards glycopeptides and easy enrichment process. In this work, core-shell structured magnetic metal-organic framework (MOF) composites with abundant boronic acid groups were designed and synthesized for selective glycopeptide enrichment based on BAAC. The as-prepared core-shell structured magnetic MOF composites (denoted as Fe₃O₄@PVP/PEI@MOF (B)) inherited strong magnetic responsiveness from the Fe₃O₄ core as well as ultrahigh surface area and abundant boronic acid sites from the MOF shell. The affinity between boronic acid and cis-diols groups endowed the composites with improved sensitivity (0.5 fmol/μL) and selectivity (1:100) towards glycopeptides, achieving remarkable results in glycopeptides detection from standard glycoprotein digests as well as complex bio-samples. As a result, a total of 209 N-glycosylation peptides from 89 different glycoproteins were identified from human serum digests, indicating its broad prospect in glycoproteome study.

Keywords: Boronic acid; Enrichment; Glycopeptide; MOF; Mass spectrometry.

MeSH terms

Boronic Acids / chemistry*
Chemistry Techniques, Analytical / methods*
Chromatography, Affinity*
Glycopeptides / analysis*
Glycopeptides / chemistry
Humans
Magnetics
Metal-Organic Frameworks / chemistry*

Substances

Boronic Acids
Glycopeptides
Metal-Organic Frameworks