Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria

Microb Pathog. 2018 Apr:117:68-72. doi: 10.1016/j.micpath.2018.02.008. Epub 2018 Feb 7.

Abstract

The present study elucidates an eco-friendly method for synthesizing silver nanoparticles using Phenerochaete chrysosporium (MTCC-787), its bactericidal and cytotoxic effect were studied. The formation of nanoparticles was evidenced by color change and UV-Vis spectroscopy. Atomic Force Microscope and Transmission electron microscope, showed spherical and oval shapes particles in the sizes ranging between 34 and 90 nm. The biosynthesised silver nanoparticles showed significant antibacterial activity against Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis at a high dose. Further, the nanoparticles observed to be non-toxic at 12.5 μg/ml towards fibroblast cells.

Keywords: Antibacterial activity; Human pathogen; Phenerochaete chrysosporium; Silver nanoparticles.

MeSH terms

  • Anti-Bacterial Agents / chemical synthesis*
  • Anti-Bacterial Agents / pharmacology*
  • Bacteria / drug effects*
  • Basidiomycota / metabolism*
  • Fibroblasts / drug effects
  • Humans
  • Klebsiella pneumoniae / drug effects
  • Metal Nanoparticles / chemistry*
  • Microbial Sensitivity Tests
  • Particle Size
  • Pseudomonas aeruginosa / drug effects
  • Silver / chemistry*
  • Staphylococcus aureus / drug effects
  • Staphylococcus epidermidis / drug effects

Substances

  • Anti-Bacterial Agents
  • Silver