Tribromophenol affects the metabolism, proliferation, migration and multidrug resistance transporters activity of murine melanoma cells B16F1

Toxicol In Vitro. 2018 Aug:50:40-46. doi: 10.1016/j.tiv.2018.02.005. Epub 2018 Feb 8.

Abstract

Murine melanoma cells B16F1 were exposed to the flame retardant and wood preservative chemical 2,4,6-tribromophenol (TBP) during 24 and 48 h, at the concentrations found in human diet. TBP-exposed cells had increased MTT and Alamar blue® metabolism and ABCB5 mRNA levels (qPCR), but the cells had decreased proliferation (crystal violet assay), migration (scratch assay), and drug-effux transporters activity (rhodamine B efflux assay). Exposure to TBP did not affect the cell viability (neutral red and annexin V-PI assays), colony formation (colony number, clonogenic assay), and the levels of reactive oxygen species (DCF probe) or P53 mRNA (qPCR). The tested TBP concentrations had low toxicity to melanoma cells B16F1. However, dual effect on metastatic profile and chemoresistance suggests that the increase of ABCB5 positively modulates the cell chemoresistance, but decreases cell migration and proliferation. These findings may be explored in cancer therapy.

Keywords: B16F1; MDR; Melanoma; Migration; Therapy; Tribromophenol.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP-Binding Cassette Transporters / genetics
  • Animals
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Flame Retardants / toxicity*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Melanoma, Experimental* / genetics
  • Melanoma, Experimental* / metabolism
  • Melanoma, Experimental* / pathology
  • Mice
  • Phenols / toxicity*
  • Reactive Oxygen Species / metabolism
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • ABCB5 protein, mouse
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP-Binding Cassette Transporters
  • Flame Retardants
  • Phenols
  • Reactive Oxygen Species
  • Tumor Suppressor Protein p53
  • 2,4,6-tribromophenol