Polydopamine-based functional composite particles for tumor cell targeting and dual-mode cellular imaging

Yalei Zhou; Jie Zhou; Feng Wang; Haifeng Yang

doi:10.1016/j.talanta.2018.01.003

Polydopamine-based functional composite particles for tumor cell targeting and dual-mode cellular imaging

Talanta. 2018 May 1:181:248-257. doi: 10.1016/j.talanta.2018.01.003. Epub 2018 Jan 11.

Authors

Yalei Zhou¹, Jie Zhou¹, Feng Wang², Haifeng Yang³

Affiliations

¹ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China.
² The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: wangfeng@shnu.edu.cn.
³ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: hfyang@shnu.edu.cn.

PMID: 29426509
DOI: 10.1016/j.talanta.2018.01.003

Abstract

Particles which bear tumor cell targeting and multimode imaging capabilities are promising in tumor diagnosis and cancer therapy. A simple and versatile method to fabricate gold/polydopamine-Methylene Blue@Bovine Serum Albumin-glutaraldehyde-Transferrin composite particles (Au/PDA-MB@BSA-GA-Tf NPs) for tumor cell targeting and fluorescence (FL) / surface-enhanced Raman scattering (SERS) dual-modal imaging were reported in this work. Polydopamine (PDA) spheres played an important role in gold ion reduction, gold nanoparticle (Au NPs) binding and methylene blue (MB) adsorption, MB were employed as both fluorescence label and Raman reporter. In addition, glutaraldehyde (GA) crosslinked bovine serum albumin (BSA) in the outer layer of Au/PDA-MB nanoparticles can prevent MB from dissociation and leakage. The composite nanoparticles were further conjugated with transferrin (Tf) to target transferrin receptor (TfR)-overexpressed cancer cells. The targeting ability as well as the intracellular location of the probe was investigated through SERS mapping and fluorescence imaging. Their excellent biocompatibility was demonstrated by low cytotoxicity against breast cancer cell (4T1 cell).

Keywords: Au/PDA-MB@BSA-GA-Tf NPs; Biocompatibility; Fluorescence; Imaging; SERS; Tumor cell targeting.

MeSH terms

Animals
Breast Neoplasms / diagnostic imaging
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cattle
Cell Line, Tumor
Cell Survival / drug effects
Glutaral / chemistry
Gold / chemistry*
Indoles / chemistry*
Metal Nanoparticles / administration & dosage
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Methylene Blue / chemistry
Mice
Microscopy, Confocal / methods
Polymers / chemistry*
Receptors, Transferrin / chemistry
Receptors, Transferrin / metabolism
Serum Albumin, Bovine / chemistry
Spectrum Analysis, Raman / methods
Transferrin / chemistry*
Transferrin / metabolism

Substances

Indoles
Polymers
Receptors, Transferrin
Transferrin
polydopamine
Serum Albumin, Bovine
Gold
Glutaral
Methylene Blue