Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Valerie Migeot; Damien Hermand

doi:10.1007/978-1-4939-7546-4_3

Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Methods Mol Biol. 2018:1721:25-34. doi: 10.1007/978-1-4939-7546-4_3.

Authors

Valerie Migeot¹, Damien Hermand²

Affiliations

¹ URPHYM-GEMO, The University of Namur, Namur, Belgium.
² URPHYM-GEMO, The University of Namur, Namur, Belgium. Damien.Hermand@unamur.be.

PMID: 29423844
DOI: 10.1007/978-1-4939-7546-4_3

Abstract

The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

Keywords: Chromatin; Fission yeast; Histone; Modifications; Polymerase.

MeSH terms

Acetylation
Chromatin Immunoprecipitation / methods*
DNA Methylation / physiology*
Genome, Fungal*
Polymerase Chain Reaction / methods*
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Ubiquitination / physiology*