CSF Tau supplements 14-3-3 protein detection for sporadic Creutzfeldt-Jakob disease diagnosis while transitioning to next generation diagnostics

Qiao-Xin Li; Shiji Varghese; Shannon Sarros; Christiane Stehmann; James D Doecke; Christopher J Fowler; Colin L Masters; Steven J Collins

doi:10.1016/j.jocn.2018.01.064

CSF Tau supplements 14-3-3 protein detection for sporadic Creutzfeldt-Jakob disease diagnosis while transitioning to next generation diagnostics

J Clin Neurosci. 2018 Apr:50:292-293. doi: 10.1016/j.jocn.2018.01.064.

Authors

Qiao-Xin Li¹, Shiji Varghese¹, Shannon Sarros², Christiane Stehmann², James D Doecke³, Christopher J Fowler¹, Colin L Masters², Steven J Collins⁴

Affiliations

¹ National Dementia Diagnostics Laboratory, Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville 3010, Australia.
² National Dementia Diagnostics Laboratory, Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville 3010, Australia; ANCJDR, Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville 3010, Australia.
³ CSIRO Health and Biosecurity, Brisbane, QLD 4029, Australia.
⁴ National Dementia Diagnostics Laboratory, Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville 3010, Australia; ANCJDR, Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville 3010, Australia; Department of Medicine (RMH), The University of Melbourne, Parkville 3010, Australia. Electronic address: stevenjc@unimelb.edu.au.

PMID: 29422367
DOI: 10.1016/j.jocn.2018.01.064

Abstract

The pre-mortem clinical diagnosis of Creutzfeldt-Jakob disease (CJD) is supported by biomarkers, especially cerebrospinal fluid (CSF) 14-3-3 and total tau (Tau) protein levels. These CSF biomarkers have proven the most useful prior to transitioning to powerful next generation diagnostics employing protein amplification techniques such as the real time quaking-induced conversion (RT-QuIC) assay. To enhance national diagnostic capacity while transitioning to RT-QuIC assays an optimized CSF Tau cutoff was determined and shown to usefully supplement 14-3-3 protein detection.

Keywords: 14-3-3; CJD; CSF; Tau.

MeSH terms

14-3-3 Proteins / cerebrospinal fluid*
Biomarkers / cerebrospinal fluid*
Female
Humans
Sensitivity and Specificity
tau Proteins / cerebrospinal fluid*

Substances

14-3-3 Proteins
Biomarkers
tau Proteins