Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications

Maija-Liisa Mattinen; Juan José Valle-Delgado; Timo Leskinen; Tuomas Anttila; Guillaume Riviere; Mika Sipponen; Arja Paananen; Kalle Lintinen; Mauri Kostiainen; Monika Österberg

doi:10.1016/j.enzmictec.2018.01.005

Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications

Enzyme Microb Technol. 2018 Apr:111:48-56. doi: 10.1016/j.enzmictec.2018.01.005. Epub 2018 Jan 10.

Authors

Affiliations

¹ Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: maija-liisa.mattinen@aalto.fi.
² Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: juanjose.valledelgado@aalto.fi.
³ Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: timo.leskinen@aalto.fi.
⁴ Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: tuomas.t.anttila@aalto.fi.
⁵ Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: guillaume.riviere@aalto.fi.
⁶ Bioproduct Chemistry, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16300, FI-00076 Aalto, Espoo, Finland. Electronic address: mika.sipponen@aalto.fi.
⁷ VTT Technical Research Centre of Finland Ltd, P.O. Box 1000, FI-02044 VTT, Espoo, Finland. Electronic address: arja.paananen@vtt.fi.
⁸ Biohybrid Materials, Department of Bioproducts and Biosystems, School of Chemical Technology, Aalto University, P.O. Box 16100, FI-00076 Aalto, Espoo, Finland. Electronic address: kalle.lintinen@aalto.fi.
⁹ Biohybrid Materials, Department of Bioproducts and Biosystems, School of Chemical Technology, Aalto University, P.O. Box 16100, FI-00076 Aalto, Espoo, Finland. Electronic address: mauri.kostiainen@aalto.fi.

PMID: 29421036
DOI: 10.1016/j.enzmictec.2018.01.005

Abstract

Cross-linked and decolorized lignin nanoparticles (LNPs) were prepared enzymatically and chemically from softwood Kraft lignin. Colloidal lignin particles (CLPs, ca. 200 nm) in a non-malodorous aqueous dispersion could be dried and redispersed in tetrahydrofuran (THF) or in water retaining their stability i.e. spherical shape and size. Two fungal laccases, Trametes hirsuta (ThL) and Melanocarpus albomyces (MaL) were used in the cross-linking reactions. Reactivity of ThL and MaL on Lignoboost™ lignin and LNPs was confirmed by high performance size exclusion chromatography (HPSEC) and oxygen consumption measurements with simultaneous detection of red-brown color due to the formation of quinones. Zeta potential measurements verified oxidation of LNPs via formation of surface-oriented carboxylic acid groups. Dynamic light scattering (DLS) revealed minor changes in the particle size distributions of LNPs after laccase catalyzed radicalization, indicating preferably covalent intraparticular cross-linking over polymerization. Changes in the surface morphology of laccase treated LNPs were imaged by atomic force (AFM) and transmission emission (TEM) microscopy. Furthermore, decolorization of LNPs without degradation was obtained using ultrasonication with H₂O₂ in alkaline reaction conditions. The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

Keywords: Chemical oxidation; Cross-linking; Decolorization; Laccase; Lignin nanoparticle; Stabilization.

MeSH terms

Ascomycota / enzymology
Biocompatible Materials / chemistry
Biocompatible Materials / metabolism*
Colloids
Color
Cross-Linking Reagents
Fungal Proteins / metabolism
Laccase / metabolism
Lignin / chemistry
Lignin / metabolism*
Nanoparticles / chemistry*
Nanoparticles / metabolism*
Nanoparticles / ultrastructure
Nanotechnology
Oxidation-Reduction
Trametes / enzymology

Substances

Biocompatible Materials
Colloids
Cross-Linking Reagents
Fungal Proteins
Lignin
Laccase