Chemometric methods using stable isotopes and elemental fingerprinting were used to characterize organically grown rice from green and conventionally grown rice in experimental field trials in China. Carbon, nitrogen, hydrogen, and oxygen stable isotopes as well as 26 other elements were determined. Organic rice was found to be more depleted in 13C than green or conventionally grown rice because of the uptake of enriched 13C from carbon dioxide and methane respiring bacteria and more enriched in 15N because of the volatilization of the nitrogen from the urea and ammonium of the animal manures used to manufacture the organic composts. Chemometrics (principal-component analysis and linear-discriminant analysis) were used to separate the three farming methods and provided a promising scientific tool to authenticate the farming methods of different rice cultivars fertilized with animal manures, green composts, and synthetic fertilizers in China or elsewhere.
Keywords: chemometrics; linear-discriminant analysis (LDA); multielement; organic; rice; stable isotope.