MELK is a novel therapeutic target in high-risk neuroblastoma

Shan Guan; Jiaxiong Lu; Yanling Zhao; Yang Yu; Hui Li; Zhenghu Chen; Zhongcheng Shi; Haoqian Liang; Mopei Wang; Kevin Guo; Xiangmei Chen; Wenjing Sun; Shayahati Bieerkehazhi; Xin Xu; Surong Sun; Saurabh Agarwal; Jianhua Yang

doi:10.18632/oncotarget.23515

MELK is a novel therapeutic target in high-risk neuroblastoma

Oncotarget. 2017 Dec 20;9(2):2591-2602. doi: 10.18632/oncotarget.23515. eCollection 2018 Jan 5.

Authors

Shan Guan^{1

2}, Jiaxiong Lu², Yanling Zhao², Yang Yu², Hui Li^{3

4}, Zhenghu Chen², Zhongcheng Shi², Haoqian Liang², Mopei Wang^{3

5}, Kevin Guo², Xiangmei Chen^{3

6}, Wenjing Sun^{2

7}, Shayahati Bieerkehazhi³, Xin Xu², Surong Sun¹, Saurabh Agarwal², Jianhua Yang²

Affiliations

¹ Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
² Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA.
³ Department of Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
⁴ Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
⁵ Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100083, China.
⁶ Peking University Health Science Center, Beijing 100083, China.
⁷ Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China.

Abstract

Maternal embryonic leucine zipper kinase (MELK) is known to modulate intracellular signaling and control cellular processes. However, the role of MELK in oncogenesis is not well defined. In this study, using two microarray datasets of neuroblastoma (NB) patients, we identified that MELK expression is significantly correlated to poor overall survival, unfavorable prognosis, and high-risk status. We found that MELK is a direct transcription target of MYCN and MYC in NB, and MYCN increases MELK expression via direct promoter binding. Interestingly, knockdown of MELK expression significantly reduced the phosphorylation of target protein Retinoblastoma (pRb) and inhibited NB cell growth. Furthermore, pharmacological inhibition of MELK activity by small-molecule inhibitor OTSSP167 significantly inhibited cell proliferation, anchorage-independent colony formation, blocked cell cycle progression, and induced apoptosis in different NB cell lines including a drug-resistant cell line. Additionally, OTSSP167 suppressed NB tumor growth in an orthotopic xenograft mouse model. Overall, our data suggest that MELK is a novel therapeutic target for NB and its inhibitor OTSSP167 is a promising drug for further clinical development.

Keywords: MELK; MYCN/MYC; OTSSP167; chemotherapy; neuroblastoma.