Does C60 fullerene act as a transporter of small aromatic molecules?

Agnieszka Borowik; Yuriy Prylutskyy; Łukasz Kawelski; Olena Kyzyma; Leonid Bulavin; Oleksandr Ivankov; Vsevolod Cherepanov; Dariusz Wyrzykowski; Rajmund Kaźmierkiewicz; Grzegorz Gołuński; Anna Woziwodzka; Maxim Evstigneev; Uwe Ritter; Jacek Piosik

doi:10.1016/j.colsurfb.2018.01.026

Does C₆₀ fullerene act as a transporter of small aromatic molecules?

Colloids Surf B Biointerfaces. 2018 Apr 1:164:134-143. doi: 10.1016/j.colsurfb.2018.01.026. Epub 2018 Feb 3.

Affiliations

¹ Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
² Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine.
³ LABSOFT Sp. z o.o., Puławska 469, Warsaw, Poland.
⁴ Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine; Joint Institute for Nuclear Research, 141980 Dubna, Moscow Region, Russia.
⁵ Institute of Physics of NAS of Ukraine, Nauky Aven. 46, 03680 Kyiv, Ukraine.
⁶ Faculty of Chemistry, University of Gdansk, Gdańsk, Poland.
⁷ Laboratory of Biomolecular Systems Simulations, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
⁸ Department of Physics, Sevastopol State University, Sevastopol, Crimea, Russia.
⁹ Technical University of Ilmenau, Institute of Chemistry and Biotechnology, 25 Weimarer Str., 98693 Ilmenau, Germany.
¹⁰ Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland. Electronic address: jacek.piosik@biotech.ug.edu.pl.

PMID: 29413590
DOI: 10.1016/j.colsurfb.2018.01.026

Abstract

C₆₀ fullerene is reported to directly interact with biomolecules, such as aromatic mutagens or anticancer drugs. Therefore, it is extensively studied for its potential application in the fields of drug delivery and chemoprevention. Understanding the nature of fullerene-drugs interactions might contribute to optimization and modification of the existing chemotherapy systems. Possible interactions between ICR-191, a model acridine mutagen, with well-established biophysical properties and mutagenic activity, and C₆₀ fullerene aqueous solution were investigated by broad range of biophysical methods, such as Dynamic Light Scattering, Isothermal Titration Calorimetry, and Atomic Force Microscopy. Additionally, to determine biological activity of ICR-191-C₆₀ fullerene mixtures, Ames mutagenicity test was employed. It was demonstrated that C₆₀ fullerene interacts non-covalently with ICR-191 and has strong affinity to bacterial membranes. The obtained results provide practical insight into C₆₀ fullerene interactions with aromatic compounds.

Keywords: AFM; Direct interactions; Nanocarriers; Nanoparticles.

MeSH terms

Aminacrine / analogs & derivatives
Aminacrine / metabolism
Biological Transport
Biophysical Phenomena
Fullerenes / chemistry*
Hydrocarbons, Aromatic / metabolism*
Microscopy, Atomic Force
Models, Molecular
Mutagens / toxicity
Nitrogen Mustard Compounds / metabolism
Salmonella typhimurium / drug effects

Substances

Fullerenes
Hydrocarbons, Aromatic
Mutagens
Nitrogen Mustard Compounds
Aminacrine
acridine half-mustard
fullerene C60