Colorimetric aptasensors for determination of tobramycin in milk and chicken eggs based on DNA and gold nanoparticles

Abstract

Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue. The developed aptasensors showed a high selectivity and sensitivity for TOB detection. The linearity range and the detection limit were 40-200 nM and 23.3 nM respectively. The validity of the procedure and applicability of aptasensors were successfully used to detect TOB in milk and chicken eggs, and the results were excellent in accord with the values obtained by spectrofluorimetric detection.

Keywords: Aptasensor; Chloroauric acid (PubChem CID: 28133); Colorimetric; Food products; Kanamycin (PubChem CID: 6032); Streptomycin (PubChem CID: 19649); Sulfadimethoxine (PubChem CID: 5323); Sulfamethoxazole (PubChem CID: 5329); Tobramycin; Tobramycin (PubChem CID: 36294); Trichloroacetic acid (PubChem CID: 6421); UV–Vis spectrophotometer; l-Alanine (PubChem CID: 5950); l-Arginine (PubChem CID: 6322); l-Lysine (PubChem CID: 5962); l-Proline (PubChem CID: 145742); l-Tryptophan (PubChem CID: 6305); l-Tyrosine (PubChem CID: 6057).