Carotenoid and superoxide dismutase are the most effective antioxidants participating in ROS scavenging in phenanthrene accumulated wheat leaf

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are a kind of pollutants which could stimulate stress reaction in plant cells. In this study, we systematically verify that PAHs could induce an oxidative stress in plants, and describe their damages on wheat leaf subcellular structure and organelle, together with the contributions of antioxidants working against reactive oxygen species. The observation of transmission electron microscope exhibits that cell structures become plasmolyzed and distorted, and organelles disappear under phenanthrene (a model PAH) treatments. Osmiophilic granules arise with increasing phenanthrene concentrations, displaying the evidence for oxidative stress. As more H₂O₂ produce, and the accumulation of H₂O₂ is a fatal reason for cell death under PAH treatments. Through cluster analysis, Pearson correlation coefficient, principal component analysis and redundancy analysis, carotenoid and superoxide dismutase are the two most effective antioxidants to scavenge superoxide radicals among nine major antioxidants (ascorbate, glutathione, polyamines, α-tocopherol, carotenoid, catalases, ascorbate peroxidase, superoxide dismutase and glutathione-S-transferase), glutathione-S-transferase is a potential antioxidant, and Asa-GSH cycle would turn active under higher phenanthrene treatments. Ascorbate peroxidase and α-tocopherol would cause leaf moisture increase. Thus, this work provides better comprehension on the antioxidant performances and their potential application to improving plants' resistance under PAH pollution in the environment.

Keywords: Antioxidants; Plant cells; Polycyclic aromatic hydrocarbons; Reactive oxygen species; Statistical analysis.