Volatile components of Rhizoma Alpiniae Officinarum using three different extraction methods combined with gas chromatography-mass spectrometry

Zhi-Sheng Xie; Xin-Jun Xu; Chun-Yan Xie; Jie-Yun Huang; Mei Yang; De-Po Yang

doi:10.1016/j.jpha.2012.09.005

Volatile components of Rhizoma Alpiniae Officinarum using three different extraction methods combined with gas chromatography-mass spectrometry

J Pharm Anal. 2013 Jun;3(3):215-220. doi: 10.1016/j.jpha.2012.09.005. Epub 2012 Oct 7.

Authors

Zhi-Sheng Xie^{1

2}, Xin-Jun Xu^{1

2}, Chun-Yan Xie³, Jie-Yun Huang^{1

2}, Mei Yang^{1

2}, De-Po Yang^{1

2}

Affiliations

¹ School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
² Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China.
³ Hainan Bright Future Pharmaceutical Co., Ltd. Haikou 570216, China.

Abstract

Volatile components from Rhizoma Alpiniae Officinarum were respectively extracted by three methods including hydrodistillation, headspace solid-phase microextraction (HS-SPME) and diethyl ether extraction. A total of 40 (hydrodistillation), 32 (HS-SPME) and 37 (diethyl ether extraction) compounds were respectively identified by gas chromatography-mass spectrometry (GC/MS) and 22 compounds were overlapped, including α-farnesene, γ-muurolene, 2,6-dimethyl-6-(4-methyl-3-pentenyl)bicyclo[3.1.1]hept-2-ene, eucalyptol and cadina-1(10), 4-diene and so forth, varying in relative contents. HS-SPME is fast, sample saving and solvent-free and it also can achieve similar profiles as those from hydrodistillation and solvent extraction. Therefore, it can be the priority for extracting volatile components from medicinal plants.

Keywords: Diethyl ether extraction; Gas chromatography–mass spectrometry; Headspace solid-phase microextraction; Hydrodistillation; Rhizoma Alpiniae Officinarum; Volatile components.