Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

Qian-Yun Zhang; Hui Chen; Zhen Lin; Jin-Ming Lin

doi:10.1016/j.jpha.2011.10.001

Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

J Pharm Anal. 2012 Apr;2(2):130-135. doi: 10.1016/j.jpha.2011.10.001. Epub 2012 Jan 27.

Authors

Qian-Yun Zhang^{1

2}, Hui Chen¹, Zhen Lin¹, Jin-Ming Lin¹

Affiliations

¹ Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100029, China.
² Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Abstract

A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better linearity, recovery, precision, sensitivity and validity. AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA, and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit. The correlation coefficient between MPs-CLEIA and ELISA was obtained with R²=0.6703; however, the correlation between MPs-CLEIA and ECLIA (R²=0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R²=0.6866).

Keywords: Chemiluminescence enzyme immunoassay; Colorimetric enzyme-linked immunosorbent assay; Hepatocellular carcinoma; Magnetic microparticles; α-Fetoprotein.