A new polyketide purpurogenic acid: the activated production of polyketides by the diethyl sulphate mutagenesis of marine-derived Penicillium purpurogenum G59

Nat Prod Res. 2019 Jan;33(1):89-94. doi: 10.1080/14786419.2018.1434644. Epub 2018 Feb 4.

Abstract

A new polyketide, purpurogenic acid (1), and two known polyketides, (-)-mitorubrin (2) and (-)-mitorubrinol (3), were isolated from a fungal mutant derived from the diethyl sulphate (DES) mutagenesis of marine-derived Penicillium purpurogenum G59. The planar structure of new 1 was elucidated by spectroscopic methods and the absolute configuration was assigned on the basis of [α]D and CD data. In our preliminary MTT assay, 1 inhibited human cancer K562, HL-60, HeLa and BGC-823 cells with the inhibition rates of 52.7, 78.8, 38.4 and 35.3% at the 100 μg/mL, respectively.

Keywords: (-)-mutorubrin; (-)-mutorubrinol; Purpurogenic acid; diethyl sulphate (DES) mutagenesis; polyketides.

MeSH terms

  • Cell Line, Tumor
  • HL-60 Cells
  • HeLa Cells
  • Humans
  • K562 Cells
  • Molecular Structure
  • Mutagenesis*
  • Penicillium / chemistry*
  • Penicillium / genetics
  • Polyketides / chemistry
  • Polyketides / isolation & purification*
  • Stereoisomerism
  • Sulfuric Acid Esters
  • Talaromyces / chemistry*
  • Talaromyces / genetics

Substances

  • Polyketides
  • Sulfuric Acid Esters
  • diethyl sulfate