The systematic position of Dryopteris blanfordii subsp. nigrosquamosa (Ching) Fraser-Jenkins within the genus Dryopteris Adans

Anastasiya A Krinitsina; Maxim S Belenikin; Olga A Churikova; Sergey V Kuptsov; Maxim I Antipin; Maria D Logacheva; Anna S Speranskaya

doi:10.3897/phytokeys.90.14745

The systematic position of Dryopteris blanfordii subsp. nigrosquamosa (Ching) Fraser-Jenkins within the genus Dryopteris Adans

PhytoKeys. 2017 Nov 15:(90):89-112. doi: 10.3897/phytokeys.90.14745. eCollection 2017.

Authors

Anastasiya A Krinitsina¹, Maxim S Belenikin², Olga A Churikova¹, Sergey V Kuptsov¹, Maxim I Antipin³, Maria D Logacheva⁴, Anna S Speranskaya¹

Affiliations

¹ Department of High Plants, Biological Faculty, Lomonosov Moscow State University, Leninskie gory, 1, 12, Moscow, Russia, 119234.
² Department of Molecular and Biological Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, 141700.
³ Botanical Garden, Lomonosov Moscow State University, Leninskie gory, Moscow, Russia, 119899.
⁴ Department of Evolutional Biochemistry, A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie gory, 1, 40, Moscow, Russia, 119992.

Abstract

Dryopteris blanfordii (C.Hope) C.Chr. is a member of the Dryopteridaceae, growing in high altitude Picea or Abies forests (2900-3500 m) in China and India. Phylogenetic relationships between D. blanfordii subsp. nigrosquamosa and closely related species of Dryopteris were investigated using a combined analysis of multiple molecular data sets (the protein-coding region of rbcL and matK genes and intergenic spacers psbA-trnH, trnP-petG, rps4-trnS, trnL-trnF and rbcL-accD). An assumption about the position of D. blanfordii subsp. nigrosquamosa within Dryopteris was made by using the Maximum Likelihood and Bayesian Inference approach and chloroplast marker sequences of Dryopteris species from GenBank. The results demonstrated that Asian taxa D. blanfordii subsp. nigrosquamosa and D. laeta as well as two American species D. arguta and D. marginalis belong to the same clade, all four of them being part of Dryopteris section Dryopteris.

Keywords: Dryopteridaceae; Dryopteris blanfordii subsp. nigrosquamosa; chloroplast genome markers; phylogeny.