Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee

Benjamin Dainat; Daniela Grossar; Brigitte Ecoffey; Christoph Haldemann

doi:10.1016/j.mimet.2018.01.018

Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee

J Microbiol Methods. 2018 Mar:146:61-63. doi: 10.1016/j.mimet.2018.01.018. Epub 2018 Jan 31.

Authors

Benjamin Dainat¹, Daniela Grossar², Brigitte Ecoffey³, Christoph Haldemann³

Affiliations

¹ Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland. Electronic address: benjamin.dainat@agroscope.admin.ch.
² Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland; Department of Ecology and Evolution, University of Lausanne, Biophore, UNIL-Sorge, CH-1015 Lausanne, Switzerland.
³ Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland.

PMID: 29391178
DOI: 10.1016/j.mimet.2018.01.018

Abstract

The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.

Keywords: Apis mellifera; Diagnosis; Melissococcus plutonius; Multiplex real-time PCR; Paenibacillus larvae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Americas
Animal Diseases / diagnosis*
Animal Diseases / microbiology*
Animals
Bees / genetics
Bees / microbiology*
DNA, Bacterial / genetics
Enterococcaceae / genetics
Enterococcaceae / isolation & purification
Enterococcaceae / pathogenicity
Europe
Multiplex Polymerase Chain Reaction / methods*
Multiplex Polymerase Chain Reaction / veterinary*
Paenibacillus larvae / genetics
Paenibacillus larvae / isolation & purification
Paenibacillus larvae / pathogenicity

Substances

DNA, Bacterial