The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.
Keywords: Apis mellifera; Diagnosis; Melissococcus plutonius; Multiplex real-time PCR; Paenibacillus larvae.
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