Convenient isolation of strictinin-rich tea polyphenol from Chinese green tea extract by zirconium phosphate

J Food Drug Anal. 2018 Jan;26(1):100-106. doi: 10.1016/j.jfda.2016.11.013. Epub 2017 Feb 21.

Abstract

Zirconium phosphate (ZrP) was prepared and employed to separate strictinin-rich tea polyphenol from Chinese green tea extracts. The influences of ZrP calcination temperatures, green tea extraction conditions, and the amounts of ZrP on the isolation of strictinin-rich tea polyphenol were evaluated; the absorption and desorption dynamics of strictinin on ZrP were also determined. Our results revealed that the HPLC content of strictinin increased from 4.96% in 70% ethanol extract of green tea to 58.2% in isolated strictinin-rich tea polyphenol obtained by ZrP-900 (ZrP calcined at 900°C). Furthermore, the suitable time for both strictinin absorption and desorption was 4 hours at 37°C. The method developed here consisted of easy steps such as ZrP absorption, water washing, and 0.4% phosphoric acid solution desorption, which may facilitate the detection and isolation of strictinin from different samples.

Keywords: green tea; separation; strictinin; zirconium phosphate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Mass Spectrometry
  • Molecular Structure
  • Phenols / chemistry*
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification*
  • Polyphenols / chemistry*
  • Polyphenols / isolation & purification*
  • Tea / chemistry*
  • Zirconium* / chemistry

Substances

  • Phenols
  • Plant Extracts
  • Polyphenols
  • Tea
  • strictinin
  • zirconium phosphate
  • Zirconium

Grants and funding

This work was financially supported by the Natural Science Foundation of Anhui Province (No. 1608085QH178), Anhui Key Project of Science and Technology (No. 1401032006) and Doctoral Start-up Research Fund of Xuancheng Campus of Hefei University of Technology (No. XC2015JZBZ02).