Peroxisome proliferator-activated receptor beta (PPARβ) is a ligand-activated transcription factor that plays critical roles in the regulation of many important physiological processes. In this study, PPARβ was cloned and characterized in yellow catfish Pelteobagrus fulvidraco. PPARβ cDNA was 2350 bp in length with an open reading frame (ORF) of 1530 bp, encoding 509 amino acids, a 5'-untranslated region (UTR) of 474 bp, and a 3'-UTR of 346 bp. Similar to mammals, PPARβ protein was predicted to consist of four domains, the A/B domain, DNA-binding domain (DBD), D domain, and ligand-binding domain (LBD). The DBD contained two zinc fingers with eight conserved cysteine residues. The predicted secondary structure of LBD consisted of 12 highly conserved α-helices and a small β-sheet of 4 strands. In addition, PPARβ was widely expressed across the tested tissues (liver, heart, muscle, intestine, brain, spleen, kidney, fat, ovary, and gill), but at the variable levels. Furthermore, the transcriptional responses of PPARβ by dietary Cu and Zn levels were also investigated. Dietary Cu levels showed no significant effects on PPARβ mRNA levels in the liver and intestine; in contrast, dietary Zn levels upregulated the hepatic PPARβ mRNA levels, but not in the intestine. The present study serves to increase our understanding into the function of the PPARβ gene in fish.
Keywords: Cu; Molecular characterization; PPARβ; Pelteobagrus fulvidraco; Tissue expression; Transcriptional regulation; Zn.