Abstract
Juniperus chinensis, commonly Chinese juniper, has been used for treating inflammatory diseases. This study aimed to investigate anti-atopic dermatitis (AD) effects of standardized J. chinensis fruits extract on murine oxazolone- and 2,4-dinitrochlorobenzene (DNCB)-induced models of AD. Ear swelling, epidermis thickening, and eosinophils infiltration in the oxazolone-mediated dermatitis of BALB/c mice were significantly reduced upon topical application of J. chinensis fruits 95% EtOH extract (JCE). Besides, transdermal administration of JCE to SKH-1 hairless mice inhibited the development of DNCB-induced AD-like skin lesions by suppressing transepidermal water loss and improving skin hydration. Decreased total serum immunoglobulin E (IgE) and interleukin (IL)-4 levels could be observed in atopic dorsal skin samples of JCE-treated group. According to the phytochemical analysis, JCE was found to contain isoscutellarein-7-O-β-D-xyloside, cupressuflavone, and amentoflavone as main compounds. Therapeutic attempts with the J. chinensis fruits might be useful in the treatment of AD and related skin inflammatory diseases.
Keywords:
Juniperus chinensis fruit; atopic dermatitis; immunoglobulin E; interleukin 4; skin hydration; transepidermal water loss.
MeSH terms
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Adjuvants, Immunologic / toxicity
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Administration, Cutaneous
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
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Anti-Inflammatory Agents, Non-Steroidal / analysis
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
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Biflavonoids / administration & dosage
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Biflavonoids / analysis
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Biflavonoids / chemistry
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Biflavonoids / therapeutic use
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Dermatitis, Atopic / chemically induced
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Dermatitis, Atopic / immunology
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Dermatitis, Atopic / pathology
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Dermatitis, Atopic / prevention & control*
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Dinitrochlorobenzene / toxicity
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Female
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Flavonoids / administration & dosage
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Flavonoids / analysis
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Flavonoids / chemistry
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Flavonoids / therapeutic use
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Fruit / chemistry*
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Fruit / growth & development
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Glycosides / administration & dosage
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Glycosides / analysis
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Glycosides / chemistry
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Glycosides / therapeutic use
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Immunoglobulin E / analysis
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Interleukin-4 / blood
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Irritants / toxicity
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Juniperus / chemistry*
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Juniperus / growth & development
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Mice, Hairless
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Mice, Inbred BALB C
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Molecular Structure
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Oxazolone / toxicity
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / chemistry
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Plant Extracts / therapeutic use*
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Republic of Korea
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Skin / drug effects*
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Skin / immunology
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Skin / metabolism
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Skin / pathology
Substances
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Adjuvants, Immunologic
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Anti-Inflammatory Agents, Non-Steroidal
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Biflavonoids
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Dinitrochlorobenzene
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Flavonoids
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Glycosides
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Irritants
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Plant Extracts
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Oxazolone
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Interleukin-4
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Immunoglobulin E