Abstract
Interleukin (IL)-19 is a member of the IL-10 family of interleukins and is an immuno-modulatory cytokine produced by the main macrophages. The gastrointestinal tissues of IL-19 knockout mice show exacerbated experimental colitis mediated by the innate immune system and T cells. There is an increasing focus on the interaction and relationship of IL-19 with the function of T cells. Contact hypersensitivity (CHS) is T cell-mediated cutaneous inflammation. Therefore, we asked whether IL-19 causes CHS. We investigated the immunological role of IL-19 in CHS induced by 1-fluoro-2,4-dinitrofluorobenzene as a hapten. IL-19 was highly expressed in skin exposed to the hapten, and ear swelling was increased in IL-19 knockout mice. The exacerbation of the CHS response in IL-19 knockout mice correlated with increased levels of IL-17 and IL-6, but no alterations were noted in the production of interferon (IFN)γ and IL-4 in the T cells of the lymph nodes. In addition to the effect on T cell response, IL-19 knockout mice increased production of inflammatory cytokines. These results show that IL-19 suppressed hapten-dependent skin inflammation in the elicitation phase of CHS.
Keywords:
IL-17; anti-inflammatory cytokine; interleukin (IL)-19; skin inflammation.
MeSH terms
-
Animals
-
Cells, Cultured
-
Dermatitis, Contact / blood
-
Dermatitis, Contact / immunology
-
Dermatitis, Contact / metabolism*
-
Dermatitis, Contact / pathology
-
Dinitrofluorobenzene / analogs & derivatives
-
Dinitrofluorobenzene / toxicity
-
Ear
-
Gene Expression Regulation / drug effects
-
Haptens / toxicity
-
Immunity, Innate / drug effects
-
Immunohistochemistry
-
Interleukin-10
-
Interleukin-17 / agonists
-
Interleukin-17 / genetics
-
Interleukin-17 / metabolism
-
Interleukin-6 / agonists
-
Interleukin-6 / genetics
-
Interleukin-6 / metabolism
-
Interleukins / agonists*
-
Interleukins / blood
-
Interleukins / genetics
-
Interleukins / metabolism
-
Lymph Nodes / drug effects
-
Lymph Nodes / immunology
-
Lymph Nodes / metabolism*
-
Lymph Nodes / pathology
-
Mice, Inbred BALB C
-
Mice, Knockout
-
RNA, Messenger / metabolism
-
Skin / drug effects
-
Skin / immunology
-
Skin / metabolism*
-
Skin / pathology
-
Spleen / drug effects
-
Spleen / immunology
-
Spleen / metabolism
-
Spleen / pathology
-
T-Lymphocytes / drug effects
-
T-Lymphocytes / immunology
-
T-Lymphocytes / metabolism
-
T-Lymphocytes / pathology
-
Th1 Cells / drug effects
-
Th1 Cells / immunology
-
Th1 Cells / metabolism
-
Th1 Cells / pathology
Substances
-
Haptens
-
Il19 protein, mouse
-
Interleukin-17
-
Interleukin-6
-
Interleukins
-
RNA, Messenger
-
interleukin-6, mouse
-
Interleukin-10
-
Dinitrofluorobenzene