Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy

Haiying Guo; Yizhan Xing; Yiming Zhang; Long He; Fang Deng; Xiaogen Ma; Yuhong Li

doi:10.7717/peerj.4306

Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy

PeerJ. 2018 Jan 26:6:e4306. doi: 10.7717/peerj.4306. eCollection 2018.

Authors

Haiying Guo^#¹, Yizhan Xing^#¹, Yiming Zhang², Long He^{1

3}, Fang Deng¹, Xiaogen Ma¹, Yuhong Li¹

Affiliations

¹ Department of Cell Biology, Army Medical University, Chongqing, China.
² Department of Plastic and Cosmetic surgery, Xinqiao Hospital, Army Medical University, Chongqing, China.
³ "111" Project Laboratory of Biomechanics and Tissue Repair & Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

^# Contributed equally.

Abstract

Dermal papilla (DP) plays important roles in hair follicle regeneration. Long-term culture of mouse DP cells can provide enough cells for research and application of DP cells. We optimized the culture strategy for DP cells from three dimensions: stepwise dissection, collagen I coating, and optimized culture medium. Based on the optimized culture strategy, we immortalized primary DP cells with SV40 large T antigen, and established several immortalized DP cell strains. By comparing molecular expression and morphologic characteristics with primary DP cells, we found one cell strain named iDP6 was similar with primary DP cells. Further identifications illustrate that iDP6 expresses FGF7 and α-SMA, and has activity of alkaline phosphatase. During the process of characterization of immortalized DP cell strains, we also found that cells in DP were heterogeneous. We successfully optimized culture strategy for DP cells, and established an immortalized DP cell strain suitable for research and application of DP cells.

Keywords: Cell culture; Dermal papilla; Immortalization; SV40 large T antigen.

Associated data

figshare/10.6084/m9.figshare.5752476

Grants and funding

This work was supported by the National Natural Science Foundation of China (No. 81472895) and the Natural Science Foundation of Chongqing (No. cstc2015jcyjA1219). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.