Genome sequence and detection of peach rosette mosaic virus

Thien Ho; Audra Harris; Asimina Katsiani; Archana Khadgi; Annemiek Schilder; Ioannis E Tzanetakis

doi:10.1016/j.jviromet.2018.01.004

Genome sequence and detection of peach rosette mosaic virus

J Virol Methods. 2018 Apr:254:8-12. doi: 10.1016/j.jviromet.2018.01.004. Epub 2018 Jan 31.

Authors

Thien Ho¹, Audra Harris¹, Asimina Katsiani¹, Archana Khadgi¹, Annemiek Schilder², Ioannis E Tzanetakis³

Affiliations

¹ Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, United States.
² Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, United States.
³ Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, United States. Electronic address: itzaneta@uark.edu.

PMID: 29371090
DOI: 10.1016/j.jviromet.2018.01.004

Abstract

Peach rosette mosaic disease was first described in the 1940s affecting peach and plum. It was later determined that peach rosette mosaic virus (PRMV) is the causal agent of the disease. PRMV, a member of the genus Nepovirus, infects several perennial crops including stone fruit, grape and blueberry as well as several weed species found in orchards around the world. The molecular characterization of the virus is limited to partial genome sequences making it difficult to develop reliable and sensitive molecular detection tests; the reason that detection is routinely performed using ELISA with antibodies risen against a single virus isolate. Given the potential economic impact of the virus and the modes of transmission which, in addition to nematodes, include seed we studied PRMV in more depth using a modified dsRNA extraction protocol to obtain the virus genome. We determined the full nucleotide sequence and developed a protocol that detects conserved regions present in RNA 1 and RNA 2, making it an excellent alternative to the detection protocols used today.

Keywords: Certification; Detection; Nepovirus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Genome, Viral*
Genomics / methods
Mosaic Viruses / genetics
Nepovirus / genetics*
Nepovirus / isolation & purification
Plant Diseases / virology*
Polymerase Chain Reaction
RNA, Double-Stranded
RNA, Viral*

Substances

RNA, Double-Stranded
RNA, Viral