LC-MS/MS Analysis of Bile Acids

Sabrina Krautbauer; Gerhard Liebisch

doi:10.1007/978-1-4939-7592-1_8

LC-MS/MS Analysis of Bile Acids

Methods Mol Biol. 2018:1730:103-110. doi: 10.1007/978-1-4939-7592-1_8.

Authors

Sabrina Krautbauer¹, Gerhard Liebisch²

Affiliations

¹ Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
² Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany. gerhard.liebisch@ukr.de.

PMID: 29363069
DOI: 10.1007/978-1-4939-7592-1_8

Abstract

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

Keywords: Bile acids; Electrospray ionization; Internal standards; Isomer separation; Liquid chromatography; Method; Quantification; Tandem mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bile Acids and Salts / blood*
Chromatography, Liquid / methods*
Humans
Isotope Labeling
Reproducibility of Results
Tandem Mass Spectrometry / methods*

Substances

Bile Acids and Salts