Multispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser

Takashi Buma; Nicole C Conley; Sang Won Choi

doi:10.1364/BOE.9.000276

Multispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser

Biomed Opt Express. 2017 Dec 19;9(1):276-288. doi: 10.1364/BOE.9.000276. eCollection 2018 Jan 1.

Authors

Takashi Buma¹, Nicole C Conley¹, Sang Won Choi^{1

2}

Affiliations

¹ Department of Electrical, Computer, and Biomedical Engineering, Union College, Schenectady, NY 12308, USA.
² Currently with the Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Abstract

We demonstrate optical resolution photoacoustic microscopy (OR-PAM) of lipid-rich tissue between 1050-1714 nm using a pulsed supercontinuum laser based on a large-mode-area photonic crystal fiber. OR-PAM experiments of lipid-rich samples show the expected optical absorption peaks near 1210 and 1720 nm. These results show that pulsed supercontinuum lasers are promising for OR-PAM applications such as label-free histology of lipid-rich tissue and imaging small animal models of disease.

Keywords: (060.5295) Photonic crystal fibers; (110.5120) Photoacoustic imaging; (190.4370) Nonlinear optics, fibers; (320.6629) Supercontinuum generation.