How proteolytic inhibitors interact with dentin on glass-fiber post luting over 6 months

Larissa Pinceli Chaves; Thales Lippi Ciantelli; Diana F G Araújo; Marina Ciccone Giacomini; Leo Tjäderhane; Polliana Mendes Candia Scaffa; Heitor Marques Honório; Linda Wang

doi:10.1016/j.jmbbm.2018.01.011

How proteolytic inhibitors interact with dentin on glass-fiber post luting over 6 months

J Mech Behav Biomed Mater. 2018 Mar:79:348-353. doi: 10.1016/j.jmbbm.2018.01.011. Epub 2018 Jan 11.

Authors

Larissa Pinceli Chaves¹, Thales Lippi Ciantelli², Diana F G Araújo³, Marina Ciccone Giacomini², Leo Tjäderhane⁴, Polliana Mendes Candia Scaffa⁵, Heitor Marques Honório⁶, Linda Wang⁷

Affiliations

¹ Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil; Department of Operative Dentistry, Universidade Paranaense, Cascavel, PR, Brazil.
² Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
³ Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil; Department of Operative Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
⁴ Institute of Dentistry, University of Oulu, and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland; Department of Oral and Maxillofacial Diseases, University of Helsinki, and Helsinki University Hospital, Helsinki, Finland.
⁵ Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
⁶ Department of Pediatric Dentistry, Orthodontics and Collective Health Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, SP, Brazil.
⁷ Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: wang.linda@usp.br.

PMID: 29358152
DOI: 10.1016/j.jmbbm.2018.01.011

Abstract

Objectives: Enzyme inhibitors minimize the degradation of unprotected collagen of dentin promoted by matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). As the evidence of their effect on the root canal is limited, this study aimed to evaluate the role of EDTA, chlorhexidine and E-64 as antiproteolytic agents on the bond strength (BS) of glass-fiber posts in root canals.

Materials and methods: Ninety-six bovine roots were distributed in groups for each time point (n = 8). Adper Scotchbond Multipurpose (MP)/ RelyX ARC system was used to lute the post according to the treatment: negative control (NC)- water, EDTA- 17% ethylenediaminetetraacetic acid, CHX- 2% digluconate chlorhexidine, E-64-5- 5μM E-64, E-64-10- 10μM E-64 and positive control (PC)- MP associated with activator/ catalyst. Then, slices were subjected to push-out test (0.5mm/min) after 24h/6 mons. Data were analyzed by three-way ANOVA/Tukey tests. Failure modes were analyzed (40×).

Results: The factors treatment, time, root canal third and the interaction between treatment and time were statistically significant. At 24h, no negative interactions were observed among the root dentin, bonding system and post. At 6 mons, CHX improved the BS for middle and apical root thirds.

Conclusions: CHX was able to promote beneficial BS after 6 mons, which was not noted for any other tested enzyme inhibitors.

Keywords: Bond strength; Chlorhexidine; E-64; EDTA; Root canal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Dental Materials / chemistry*
Dental Stress Analysis
Dentin / chemistry*
Enzyme Inhibitors / chemistry*
Materials Testing
Tooth Root*

Substances

Dental Materials
Enzyme Inhibitors