Sulfated Laminaria japonica polysaccharides inhibit macrophage foam cell formation

Int J Biol Macromol. 2018 May:111:857-861. doi: 10.1016/j.ijbiomac.2018.01.103. Epub 2018 Feb 19.

Abstract

In this work, a purified Laminaria japonica polysaccharide (LJP61A) was chemically modified to obtain three sulfated polysaccharides (SLJP1, SLJP2 and SLJP3) with different degrees of sulfation using the method of chlorosulfonic acid/pyridine. The effects and underlying mechanism of SLJP1, SLJP2 and SLJP3 on the suppression of macrophage foam cell formation were further investigated using the model of oxidized low-density lipoprotein (ox-LDL)-induced foam cell formation. Results exhibited that the macrophage foam cell formation induced by ox-LDL could be significantly alleviated by these sulfated polysaccharides in a dose-dependent manner. Meanwhile, the enhancement of PPAR-γ mRNA expression in ox-LDL induced macrophages was remarkably inhibited by these sulfated polysaccharides. Moreover, the cellular inflammation induced by ox-LDL could also be remarkably mitigated by these sulfated polysaccharides. These results indicated that the sulfated L. japonica polysaccharides could inhibit the conversion of macrophage into foam cell via obstructing PPAR-γ activation and alleviating cellular inflammation.

Keywords: Laminaria japonica; Polysaccharide; Sulfation.

MeSH terms

  • Animals
  • Foam Cells / drug effects*
  • Gene Expression Regulation / drug effects
  • Humans
  • Inflammation / drug therapy*
  • Inflammation / genetics
  • Laminaria / chemistry*
  • Lipoproteins, LDL / genetics
  • Macrophages / drug effects
  • Mice
  • PPAR gamma / chemistry
  • PPAR gamma / genetics
  • Polysaccharides / chemistry*
  • Polysaccharides / pharmacology
  • RAW 264.7 Cells / drug effects
  • Sulfates / chemistry

Substances

  • Lipoproteins, LDL
  • PPAR gamma
  • Polysaccharides
  • Sulfates
  • oxidized low density lipoprotein