Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY

Yee Meng Wong; Ta Yeong Wu; Tau Chuan Ling; Pau Loke Show; Sze Ying Lee; Jo-Shu Chang; Shaliza Ibrahim; Joon Ching Juan

doi:10.1016/j.jbiosc.2017.12.012

Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY

J Biosci Bioeng. 2018 May;125(5):590-598. doi: 10.1016/j.jbiosc.2017.12.012. Epub 2018 Jan 17.

Authors

Yee Meng Wong¹, Ta Yeong Wu², Tau Chuan Ling³, Pau Loke Show⁴, Sze Ying Lee⁴, Jo-Shu Chang⁵, Shaliza Ibrahim⁶, Joon Ching Juan⁷

Affiliations

¹ Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia.
² Chemical and Sustainable Process Engineering Research Group, School of Engineering, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia.
³ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁴ Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia.
⁵ Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan.
⁶ Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁷ Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia; Nanotechnology and Catalysis Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: jcjuan@um.edu.my.

PMID: 29352712
DOI: 10.1016/j.jbiosc.2017.12.012

Abstract

Three newly discovered H₂ producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H₂ production. The maximum H₂ production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H₂/mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H₂/mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between -33 and -35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H₂ production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H₂ production.

Keywords: Biohydrogen; Clostridium spp.; Dark fermentation; Hydrogenase; Thermodynamics.

MeSH terms

Clostridium bifermentans / isolation & purification
Clostridium bifermentans / metabolism*
Clostridium perfringens / classification*
Clostridium perfringens / isolation & purification
Clostridium perfringens / metabolism*
Fermentation
Glucose / metabolism
Hydrogen / metabolism*
Sewage / microbiology

Substances

Sewage
Hydrogen
Glucose