To overcome drug resistance, efficient cancer therapeutic strategies using a combination of small-molecule drugs and macromolecule drugs is highly desired. However, because of their significant differences in molecular weight and size, it is difficult to load them simultaneously in one vector and to release them individually. Here, a biodegradable organosilica-based core-shell-structured nanocapsule was designed and used as a dual stimuli-responsive drug vector to solve this problem. Biodegradable organosilica shell coated outside the macromolecule model drug "core" would be disrupted by high glutathione (GSH) levels inside tumor cells, resulting in the escape of the entrapped drugs. Small-molecule drugs capping on the surface of the organosilica shell via pH-responsive imine bonds can be cut and released in the acidic lysosomal environment. Transmission electron microscopy has shown that the framework of the organosilica shell was dissolved and degraded after 8 h incubation with 5 mM GSH. Confocal imaging confirmed that small-molecule and macromolecular drugs were individually released from the nanoparticles because of the pH or redox-triggered degradation under the tumor microenvironment and thus led to the strong fluorescence recovery in the cytoplasm. As expected, these biodegradable organosilica nanoparticles could not release drugs into normal cells but could specifically release them into tumor cells owing to their tumor-triggered targeting capability. This system will serve as an efficient shuttle for multidrug delivery and also provide a potential strategy to overcome drug resistance.
Keywords: biodegradable organosilica; cancer therapy; multidrug delivery; stimuli-responsive; tumor-triggered targeting.