Diffusion Tensor Imaging is an MRI technique that allows in vivo noninvasive measurement of the translational motion of water, providing information about its anisotropy (or lack of it) in different tissues. DTI has been commonly used to quantitatively measure the integrity of tissues which may be compromised by neurological disease, such as white matter tracks of the brain, which normally impart significant anisotropy to water motion in healthy brains. However, this anisotropic effect is diminished when axonal or neuronal damage is present. This chapter describes a standard protocol for DTI data acquisition in preclinical studies.
Keywords: Apparent diffusion coefficient; Axial diffusivity; Diffusion tensor imaging; Fiber tracking; Fractional anisotropy; Radial diffusivity; White matter.