Perturbations of gut microbiome genes in infants with atopic dermatitis according to feeding type

Min-Jung Lee; Mi-Jin Kang; So-Yeon Lee; Eun Lee; Kangjin Kim; Sungho Won; Dong In Suh; Kyung Won Kim; Youn Ho Sheen; Kangmo Ahn; Bong-Soo Kim; Soo-Jong Hong

doi:10.1016/j.jaci.2017.11.045

Perturbations of gut microbiome genes in infants with atopic dermatitis according to feeding type

J Allergy Clin Immunol. 2018 Apr;141(4):1310-1319. doi: 10.1016/j.jaci.2017.11.045. Epub 2018 Jan 12.

Authors

Min-Jung Lee¹, Mi-Jin Kang², So-Yeon Lee³, Eun Lee⁴, Kangjin Kim⁵, Sungho Won⁶, Dong In Suh⁷, Kyung Won Kim⁸, Youn Ho Sheen⁹, Kangmo Ahn¹⁰, Bong-Soo Kim¹¹, Soo-Jong Hong¹²

Affiliations

¹ Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon, Korea.
² Asan Institute for Life Science, University of Ulsan College of Medicine, Seoul, Korea.
³ Department of Pediatrics, Childhood Asthma Atopy Center, Environmental Health Center, University of Ulsan College of Medicine, Seoul, Korea.
⁴ Department of Pediatrics, Chonnam National University Hospital, Gwangju, Korea.
⁵ Department of Public Health Science, Seoul National University, Seoul, Korea.
⁶ Department of Public Health Science, Seoul National University, Seoul, Korea; Institute of Health and Environment, Seoul National University, Seoul, Korea.
⁷ Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.
⁸ Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
⁹ Department of Pediatrics, CHA Gangnam Medical Center, CHA University College of Medicine, Seoul, Korea.
¹⁰ Department of Pediatrics, Environmental Health Center for Atopic Disease, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
¹¹ Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon, Korea. Electronic address: bkim79@hallym.ac.kr.
¹² Department of Pediatrics, Childhood Asthma Atopy Center, Environmental Health Center, University of Ulsan College of Medicine, Seoul, Korea. Electronic address: sjhong@amc.seoul.kr.

PMID: 29339259
DOI: 10.1016/j.jaci.2017.11.045

Abstract

Background: Perturbations of the infant gut microbiota can shape development of the immune system and link to the risk of allergic diseases.

Objective: We sought to understand the role of the gut microbiome in patients with atopic dermatitis (AD). The metagenome of the infant gut microbiome was analyzed according to feeding types.

Methods: Composition of the gut microbiota was analyzed in fecal samples from 129 infants (6 months old) by using pyrosequencing, including 66 healthy infants and 63 infants with AD. The functional profile of the gut microbiome was analyzed by means of whole-metagenome sequencing (20 control subjects and 20 patients with AD). In addition, the total number of bacteria in the feces was determined by using real-time PCR.

Results: The gut microbiome of 6-month-old infants was different based on feeding types, and 2 microbiota groups (Bifidobacterium species-dominated and Escherichia/Veillonella species-dominated groups) were found in breast-fed and mixed-fed infants. Bacterial cell amounts in the feces were lower in infants with AD than in control infants. Although no specific taxa directly correlated with AD in 16S rRNA gene results, whole-metagenome analysis revealed differences in functional genes related to immune development. The reduction in genes for oxidative phosphorylation, phosphatidylinositol 3-kinase-Akt signaling, estrogen signaling, nucleotide-binding domain-like receptor signaling, and antigen processing and presentation induced by reduced colonization of mucin-degrading bacteria (Akkermansia muciniphila, Ruminococcus gnavus, and Lachnospiraceae bacterium 2_1_58FAA) was significantly associated with stunted immune development in the AD group compared with the control group (P < .05).

Conclusions: Alterations in the gut microbiome can be associated with AD because of different bacterial genes that can modulate host immune cell function.

Keywords: Atopic dermatitis; gut; immune; infant; microbiome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Feeding*
Case-Control Studies
DNA, Bacterial
Dermatitis, Atopic / immunology
Dermatitis, Atopic / microbiology*
Feces / microbiology
Female
Gastrointestinal Microbiome / genetics*
Gastrointestinal Microbiome / immunology*
Humans
Infant
Infant Formula / adverse effects*
Male
Metagenome
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA

Substances

DNA, Bacterial