Aim: To explore the hemostatic effect of Phellodendri Cortex-derived carbon dots.
Materials & methods: Transmission electron microscopy, high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, fluorescence spectroscopy, x-ray photoelectron spectroscopy and a cell counting kit-8 assay were studied. Hemostatic effect of Phellodendri Cortex Carbonisatus-carbon dots (PCC-CDs) was studied in mouse bleeding models. To explore their related hemostatic mechanism, coagulation parameters and platelets were measured.
Results: The PCC-CDs ranged in diameter from 1.2 to 4.8 nm and had a quantum yield of 9.62%. They exhibited no toxicity up to concentrations of 1000 μg/ml. After administration, mice had a significantly shortened bleeding time and coagulation parameters and platelets significantly increased.
Conclusion: These results showed the definite hemostatic effect of PCC-CDs.
Keywords: Phellodendri Cortex Carbonisatus; bioactivity; bleeding; carbon dots; cell counting kit; coagulation parameters; cytotoxicity; hemostasis; mechanism; platelets.