Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle

Jessica Moraes Malheiros; Cruz Elena Enríquez-Valencia; Bruno Oliveira da Silva Duran; Tassiana Gutierrez de Paula; Rogério Abdallah Curi; Josineudson Augusto I I de Vasconcelos Silva; Maeli Dal-Pai-Silva; Henrique Nunes de Oliveira; Luis Artur Loyola Chardulo

doi:10.1016/j.meatsci.2018.01.003

Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle

Meat Sci. 2018 Apr:138:49-52. doi: 10.1016/j.meatsci.2018.01.003. Epub 2018 Jan 5.

Authors

Jessica Moraes Malheiros¹, Cruz Elena Enríquez-Valencia², Bruno Oliveira da Silva Duran³, Tassiana Gutierrez de Paula³, Rogério Abdallah Curi⁴, Josineudson Augusto I I de Vasconcelos Silva⁴, Maeli Dal-Pai-Silva³, Henrique Nunes de Oliveira⁵, Luis Artur Loyola Chardulo⁴

Affiliations

¹ Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP 14884-900, Brazil. Electronic address: jehmalheiros@gmail.com.
² Departamento de Ciencias Pecuarias, Facultad de Ciencias Agrarias y del Ambiente, Universidad Francisco de Paula Santander Ocaña, Norte de Santander 546552, Colombia.
³ Instituto de Biociências, Departamento de Morfologia, Universidade Estadual Paulista, Botucatu, SP 18618-689, Brazil.
⁴ Departamento de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brazil.
⁵ Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP 14884-900, Brazil.

PMID: 29331838
DOI: 10.1016/j.meatsci.2018.01.003

Abstract

The objective of this study was to evaluate the association of expression of CAPN1, CAPN2, CAST, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Three experimental groups were selected by shear force (SF): moderately tender (SF=34.3±5.8N), moderately tough (SF=56.8±7.8N), and very tough meat (SF=80.4±15N). Gene expression was evaluated by real-time PCR. Expression of the CAPN1, CAPN2, CAST and CAST1 genes did not differ between groups. Expression of the CAST2 was up-regulated (P<0.05) in the moderately tough and very tough meat groups. Down-regulation of the HSP90AA1, DNAJA1 and HSPB1 genes (P<0.05) was observed in the moderately tender meat group. The present results suggest that meat tenderness in Nellore cattle does not directly depend on the expression of the CAPN1 and CAPN2 genes, but is associated with the expression of other genes such as CAST2, HSP90AA1, DNAJA1 and HSPB1.

Keywords: Calpain complex; HSPs; RT-qPCR; Shear force.

MeSH terms

Animals
Biomarkers
Calcium-Binding Proteins / genetics*
Calcium-Binding Proteins / metabolism
Cattle / genetics*
Cattle / metabolism
Gene Expression
Male
Red Meat / analysis*
Shear Strength*

Substances

Biomarkers
Calcium-Binding Proteins