Cell wall damage and oxidative stress in Candida albicans ATCC10231 and Aspergillus niger caused by palladium nanoparticles

Toxicol In Vitro. 2018 Apr:48:111-120. doi: 10.1016/j.tiv.2018.01.006. Epub 2018 Jan 10.

Abstract

In this work the toxic effect of Palladium nanoparticles (PdNPs) was investigated in two eukaryotic cell models, Candida albicans and Aspergillus niger. PdNPs were synthesized by chemical reduction method, obtaining spherical NPs with a primary size ranging from 3 to 15 nm. PdNPs showed a hydrodynamic size of 1548 nm in Lee's minimum media. Minimal inhibitory concentration was determined at 200 and 250 ppm for Candida albicans and Aspergillus niger respectively, revealing a significant cell growth inhibition (ANOVA and tukey analysis, α = 0.5). Reactive Oxygen Species levels were increased in both microorganisms. Confocal, scanning and transmission electron microscopy studies revealed cell wall damage and cellular morphology changes, induced by the interaction of PdNPs, in both microorganisms.

Keywords: Intracellular integrity loss; Microscopy; Reactive oxygen species; Toxicity.

MeSH terms

  • Aspergillus niger / drug effects*
  • Candida albicans / drug effects*
  • Cell Wall / drug effects*
  • Cell Wall / ultrastructure
  • Metal Nanoparticles / toxicity*
  • Microbial Sensitivity Tests
  • Oxidative Stress / drug effects*
  • Palladium / toxicity*
  • Particle Size
  • Reactive Oxygen Species / metabolism

Substances

  • Reactive Oxygen Species
  • Palladium