Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Biotechnol Adv. 2018 May-Jun;36(3):641-665. doi: 10.1016/j.biotechadv.2018.01.006. Epub 2018 Jan 10.

Abstract

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

Keywords: CRISPR-Cas; Candida albicans/glabrata; Expression optimization; Metabolic engineering; Pichia pastoris; Saccharomyces cerevisiae; Schizosaccharomyces pombe; Synthetic biology; Yarrowia lipolytica; Yeasts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • CRISPR-Cas Systems*
  • Chromosomes, Fungal
  • Cloning, Molecular
  • Gene Drive Technology
  • Gene Editing / methods*
  • Gene Expression Regulation, Fungal
  • Metabolic Engineering
  • Microorganisms, Genetically-Modified
  • Pichia / genetics
  • Point Mutation
  • RNA, Guide, CRISPR-Cas Systems
  • Saccharomyces cerevisiae / genetics
  • Yarrowia / genetics
  • Yeasts / genetics*

Substances

  • RNA, Guide, CRISPR-Cas Systems