Abstract
Hypoxia (insufficient O2 availability) is involved in various biological processes, such as tumorigenesis and inflammation. Hypoxia results in stabilization of hypoxia-inducible factors (HIFs) including HIF1α and HIF2α. Here we describe a protocol to detect mouse and human tissue hypoxia by using Hypoxyprobe and immunohistochemical staining for HIF1α and HIF2α.
Keywords:
HIF1α; HIF2α; Hypoxia; Hypoxyprobe; Immunohistochemistry.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Basic Helix-Loop-Helix Transcription Factors
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Carcinoma, Pancreatic Ductal / metabolism*
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Cell Hypoxia
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
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Immunohistochemistry
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Mice
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Pancreatic Neoplasms / metabolism*
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Paraffin Embedding
Substances
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Basic Helix-Loop-Helix Transcription Factors
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HIF1A protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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endothelial PAS domain-containing protein 1