Eight Genome Sequences of Cluster BE1 Phages That Infect Streptomyces Species

Lee E Hughes; Christopher D Shaffer; Vassie C Ware; Issac Aguayo; Rahat M Aziz; Swapan Bhuiyan; Isaac S Bindert; Canyon K Calovich-Benne; Jordyn Chapman; Richard Donegan-Quick; Amal Farooq; Cynthia Garcia; Lee H Graham; Baylee Y Green; Margaret A Kenna; Emily R Kneeream; Christian E Laing; Catherine M Mageeney; Sandra N Meridew; Abigail R Mikolon; Ryann E Morgan; Subhayu Nayek; Idowu D Olugbade; Kim C Pike; Lauren E Schlegel; Taylor C Shishido; Tara Suresh; Nikita Suri; Kathleen Weston Hafer; Rebecca A Garlena; Daniel A Russell; Steven G Cresawn; Welkin H Pope; Deborah Jacobs-Sera; Graham F Hatfull

doi:10.1128/genomeA.01146-17

Eight Genome Sequences of Cluster BE1 Phages That Infect Streptomyces Species

Genome Announc. 2018 Jan 11;6(2):e01146-17. doi: 10.1128/genomeA.01146-17.

Authors

Lee E Hughes^#¹, Christopher D Shaffer^#², Vassie C Ware^#³, Issac Aguayo¹, Rahat M Aziz¹, Swapan Bhuiyan¹, Isaac S Bindert², Canyon K Calovich-Benne¹, Jordyn Chapman², Richard Donegan-Quick¹, Amal Farooq¹, Cynthia Garcia¹, Lee H Graham³, Baylee Y Green¹, Margaret A Kenna³, Emily R Kneeream⁴, Christian E Laing⁴, Catherine M Mageeney³, Sandra N Meridew¹, Abigail R Mikolon⁴, Ryann E Morgan¹, Subhayu Nayek¹, Idowu D Olugbade³, Kim C Pike³, Lauren E Schlegel³, Taylor C Shishido², Tara Suresh², Nikita Suri¹, Kathleen Weston Hafer², Rebecca A Garlena⁵, Daniel A Russell⁵, Steven G Cresawn⁶, Welkin H Pope⁵, Deborah Jacobs-Sera⁵, Graham F Hatfull⁷

Affiliations

¹ Department of Biological Sciences, University of North Texas, Denton, Texas, USA.
² Department of Biology, Washington University in St. Louis, St. Louis, Missouri, USA.
³ Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, USA.
⁴ Department of Biology, Wilkes University, Wilkes-Barre, Pennsylvania, USA.
⁵ Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
⁶ Biology Department, James Madison University, Harrisonburg, Virginia, USA.
⁷ Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA gfh@pitt.edu.

^# Contributed equally.

Abstract

Cluster BE1 Streptomyces bacteriophages belong to the Siphoviridae, with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp. Eight newly isolated closely related cluster BE1 phages contain 43 to 48 tRNAs, one transfer-messenger RNA (tmRNA), and 216 to 236 predicted open reading frames (ORFs), but few of their genes are shared with other phages, including those infecting Streptomyces species.

Grants and funding

R01 GM116884/GM/NIGMS NIH HHS/United States