Objective: To investigate the effect and mechanism of hyperbaric oxygen combined with radioactive seed implantation in the treatment of esophageal squamous cell carcinoma. Methods: Subcutaneous tumor model of esophageal squamous cell carcinoma using TE-8 cells was established. Tumor bearing Balb/c(nu/nu) mice (60 mice) were divided into four groups, Cont group that treated with normal oxygen level, HBO group that treated with hyperbaric oxygen, RSI group that treated with radioactive seed implantation, and HBO+ RSI group that treated with hyperbaric oxygen combined with radioactive seed implantation. Tumor volume ratio and mean survival time of tumor bearing mice were observed. Pathological changes of tumor tissue after treatment were observed by hematoxylin eosin (HE) staining. Enzyme linked immunosorbent assay kit was used to detect oxidative stress. Apoptosis related proteins were detected by Western blot. Results: After treatment, the tumor volume ratio of HBO+ RSI group was 3.51±0.80 and was significantly lower than that of Cont group, HBO group, and RSI group (P<0.05). The mean survival time of HBO+ RSI group tumor bearing mice was 62 d and was significantly longer than that in Cont group, HBO group, and RSI group (P<0.05). HE staining showed that the pathological changes of tumor tissues were most obvious in HBO+ RSI group. After treatment, the MDA and Bax levels in nude mice of HBO+ RSI group were significantly higher than those in Cont group, HBO group and RSI group, but the levels of GSH, SOD and Bcl-2 were significantly lower than those of Cont group, HBO group and RSI group (P<0.05). Conclusion: Hyperbaric oxygen combined with radioactive seed implantation could slow tumor growth and increase survival time of tumor bearing mice. The possible mechanism is that hyperbaric oxygen combined with radioactive seed implantation can improve the oxidative stress response and the expression of apoptosis protein in tumor bearing nude mice.
目的： 探讨高压氧与放射性粒子植入联合治疗食管鳞癌荷瘤裸鼠的治疗效果并探讨相关治疗机制。 方法： 利用TE－8细胞建立食管鳞癌皮下移植裸鼠模型，将60只荷瘤裸鼠随机平分为正常组(常压正常氧水平治疗)、高压氧治疗(HBO)组、放射性粒子植入治疗(RSI)组和HBO＋RSI组；治疗后观察食管鳞癌荷瘤裸鼠肿瘤体积比以及中位存活时间，通过苏木精－伊红(HE)染色进行肿瘤组织病理学改变检测；利用酶联免疫吸附试剂盒检测氧化应激反应；利用Western印迹检测凋亡相关蛋白。 结果： HBO＋RSI组荷瘤裸鼠经联合治疗后肿瘤体积比达到4.23±0.75，显著低于正常组、HBO组和RSI组，同时中位存活时间达到62 d，长于正常组、HBO组和RSI组，比较差异有统计学意义(P<0.05)。HE染色结果显示HBO＋RSI组肿瘤组织病理学变化最明显。联合治疗后HBO＋RSI组荷瘤裸鼠MDA和Bax水平显著高于正常组、HBO组和RSI组，而GSH、SOD和Bcl－2水平显著低于正常组、HBO组和RSI组，比较差异有统计学意义(P<0.05)。 结论： 高压氧和放射性粒子植入联合治疗减缓了食管鳞癌移植肿瘤生长速度，提高了荷瘤裸鼠存活时间，其可能原因是联合治疗改善了荷瘤裸鼠肿瘤组织氧化应激反应和凋亡蛋白表达。.
Keywords: Combined therapy; Esophageal squamous cell carcinoma; Hyperbaric oxygen; Nude mice; Radioactive seed implantation.