Bacterial branched glycerol dialkyl glycerol tetraethers (brGDGTs) have been successfully used as quantitative climate proxies for reconstructing annual mean air temperature (MAT) and soil pH from sediments. However, reconstructions derived from brGDGTs in regions with diverse hydrological and atmospheric conditions require further refinement. In this study, we investigated the suitability of brGDGTs as temperature and precipitation proxies from surface soils on opposite slopes of Mount Fanjing in subtropical China. The results show a clear altitudinal lapse rate of MBT'5ME-derived MAT and between-slope differences in MAT at given altitude. Moreover, the MBT'5ME-derived MAT values are more strongly related to the MAT from March to November than that of the whole year. A turning point is also observed from the measured pH and CBT'-derived pH gradients at altitude of 1400 m~1500 m, in accordance with the fog horizon, where precipitation reaches the maximum value. The findings prove that brGDGTs from soil transects can be used as indicators for reconstructions of climate parameters from subtropical regions of China.