Aim of the study: The aim of the study was to evaluate the effect of leukocyte- and platelet-rich plasma (L-PRP) treatment on a noncomplicated would healing after surgical skin incision in an experimental animal model.
Materials and methods: The blood from 64 male Wistar rats was used to prepare L-PRP before a transverse dorsal incision was made and consecutively closed. At the same time after the surgery was completed, autologous L-PRP in the rats from the L-PRP group (n = 32) and NaCl in the control group (n = 32) were injected subcutaneously into the wound. The examination included clinical observations, laboratory examination, and examination of biopsy specimens taken from sacrificed animals on the second, third, fourth, and sixth days after the surgery (histopathological examination, immunochemistry, molecular examination).
Results: In all cases, no healing process complications were noted. The intergroup comparison revealed no statistically significant differences in terms of histopathological, immunohistochemical, and molecular examinations results. In the intragroup analysis, statistically significant differences were observed within each group between periods of observations in terms of immunohistochemical and molecular examinations results.
Conclusions: L-PRP treatment does not significantly accelerate a noncomplicated wound healing after surgical skin incision in an experimental animal model.
Keywords: Platelet concentrate; platelet-derived growth factor; regenerative therapy; transforming growth factor beta.