Dark Septate Endophytic Fungi Help Tomato to Acquire Nutrients from Ground Plant Material

Carlos Vergara; Karla E C Araujo; Segundo Urquiaga; Nivaldo Schultz; Fabiano de Carvalho Balieiro; Peter S Medeiros; Leandro A Santos; Gustavo R Xavier; Jerri E Zilli

doi:10.3389/fmicb.2017.02437

Dark Septate Endophytic Fungi Help Tomato to Acquire Nutrients from Ground Plant Material

Front Microbiol. 2017 Dec 11:8:2437. doi: 10.3389/fmicb.2017.02437. eCollection 2017.

Authors

Carlos Vergara¹, Karla E C Araujo², Segundo Urquiaga³, Nivaldo Schultz¹, Fabiano de Carvalho Balieiro⁴, Peter S Medeiros¹, Leandro A Santos¹, Gustavo R Xavier³, Jerri E Zilli³

Affiliations

¹ Departamento de Ciências do Solo, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil.
² Departamento de Fitotecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil.
³ Embrapa Agrobiologia, Seropédica, Brazil.
⁴ Embrapa Solos, Seropédica, Brazil.

Abstract

Dark septate endophytic (DSE) fungi are facultative biotrophs that associate with hundreds of plant species, contributing to their growth. These fungi may therefore aid in the search for sustainable agricultural practices. However, several ecological functions of DSE fungi need further clarification. The present study investigated the effects of DSE fungi inoculation on nutrient recovery efficiency, nutrient accumulation, and growth of tomato plants fertilized with organic and inorganic N sources. Two experiments were carried out under greenhouse conditions in a randomized blocks design, with five replicates of tomato seedlings grown in pots filled with non-sterile sandy soil. Tomato seedlings (cv. Santa Clara I-5300) inoculated with DSE fungi (isolates A101, A104, and A105) and without DSE fungi (control) were transplanted to pots filled with 12 kg of soil which had previously received finely ground plant material [Canavalia ensiformis (L.)] that was shoot enriched with 0.7 atom % ¹⁵N (organic N source experiment) or ammonium sulfate-¹⁵N enriched with 1 atom % ¹⁵N (mineral N source experiment). Growth indicators, nutrient content, amount of nitrogen (N) in the plant derived from ammonium sulfate-¹⁵N or C. ensiformis-¹⁵N, and recovery efficiency of ¹⁵N, P, and K by plants were quantified 50 days after transplanting. The treatment inoculated with DSE fungi and supplied with an organic N source showed significantly higher recovery efficiency of ¹⁵N, P, and K. In addition, the ¹⁵N, N, P, K, Ca, Mg, Fe, Mn, and Zn content, plant height, leaf number, leaf area (only for the A104 inoculation), and shoot dry matter increased. In contrast, the only positive effects observed in the presence of an inorganic N source were fertilizer-K recovery efficiency, content of K, and leaf area when inoculated with the fungus A104. Inoculation with A101, A104, and A105 promoted the growth of tomato using organic N source (finely ground C. ensiformis-¹⁵N plant material).

Keywords: 15N; Canavalia ensiformis (L.) -15N; DSE fungi; Solanum lycopersicum (L.); nutrient recovery efficiency; phosphorus; potassium.