Antibiotic Resistance Plasmids Cointegrated into a Megaplasmid Harboring the blaOXA-427 Carbapenemase Gene

Antimicrob Agents Chemother. 2018 Feb 23;62(3):e01448-17. doi: 10.1128/AAC.01448-17. Print 2018 Mar.

Abstract

OXA-427 is a new class D carbapenemase encountered in different species of Enterobacteriaceae in a Belgian hospital. To study the dispersal of this gene, we performed a comparative analysis of two plasmids containing the blaOXA-427 gene, isolated from a Klebsiella pneumoniae strain and an Enterobacter cloacae complex strain. The two IncA/C2 plasmids containing blaOXA-427 share the same backbone; in the K. pneumoniae strain, however, this plasmid is cointegrated into an IncFIb plasmid, forming a 321-kb megaplasmid with multiple multiresistance regions.

Keywords: OXA-427; carbapenemase; megaplasmids.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Drug Resistance, Microbial / genetics
  • Enterobacter cloacae / drug effects
  • Enterobacter cloacae / genetics
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase